Anti-SSEA1 antibody [MC-480]

• Supplier: Abcam
• Catalog: ab16285
• Prices: $400.00
• Sizes: 200 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgM
• Clone: MC-480
• Applications: IHC-F ICC/IF ICC/IF IP IHC-P IHC-F FC
• Species Reactivities: Mouse
• Antigen: F9 teratocarcinoma stem cells (X-irradiated)
• Description: Mouse Monoclonal
• Gene: Fut4
• Conjugate: Unconjugated

Product images

ICC/IF image of ab16285 stained mouse embryonic stem cells. The cells were 4% formalin fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16285, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgM (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

The image shows staining of the cell membranes of P19 mouse embryonic carcinoma cells using SSEA1-specific antibody, ab16285.

Cell surface flow analysis of SSEA1 on D3 mouse ES cells using ab16285 at 1:100 dilution. Purple histogram represents negative control; green line represents anti-SSEA1 antibody (ab16285).See Abreview

IHC image of SSEA1 staining in mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16285, 10µg/ml, for 15 mins at room temperature. A Goat anti-Mouse biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

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