Anti-SSH3 antibody - C-terminal (ab170440) at 1/100 dilution + HeLa cell lysate at 35 µg
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling SSH3 with ab170440 at a 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HeLa cells labeling SSH3 with ab170440 at 1/10 dilution (right histogram), compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunofluorescent analysis of HeLa cells labeling SSH3 with ab170440 at a 1/10 dilution. Primary incubation was followed by secondary antibody labeling with Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).