Anti-SSX2IP antibody [EPR16979]

• Supplier: Abcam
• Catalog: ab199425
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16979
• Applications: IHC-P WB ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SSX2IP aa 600 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: SSX2IP
• Conjugate: Unconjugated

Product images

All lanes : Anti-SSX2IP antibody [EPR16979] (ab199425) at 1/10000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 2 : HEK293 (Human embryonic kidney) whole cell lysateLane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-SSX2IP antibody [EPR16979] (ab199425) at 1/20000 dilution + Human testis lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling SSX2IP with ab199425 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary ab.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SSX2IP with ab199425 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human kidney tissue is observed. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary ab.

SSX2IP was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with ab199425 at 1/150 dilution. Western blot was performed from the immunoprecipitate using ab199425 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Input HEK293 whole cell lysate (10 µg). Lane 2: HEK293 whole cell lysate folowing precipitation. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199425 in HEK293 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST. 10 second exposure.