Immunofluorescence analysis of A431 cells (fixative 4% paraformaldehyde) labeling STAC with ab181157 at a 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution. Counter stained with Dapi (blue).
Flow cytometry analysis of A431 cells labeling STAC using ab181157 at a 1/20 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.
All lanes : Anti-STAC antibody [EPR12805] - N-terminal (ab181157) at 1/2000 dilutionLane 1 : Human fetal brain lysateLane 2 : A431 lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-STAC antibody [EPR12805] - N-terminal (ab181157) at 1/10000 dilutionLane 1 : U87-MG lysateLane 2 : 293 lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution