Anti-Stefin B antibody

• Supplier: Abcam
• Catalog: ab53725
• Prices: $370.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ELISA IHC-P ICC/IF ICC/IF
• Species Reactivities: Human, Mouse, Rat
• Antigen: Synthetic peptide derived from human Stefin B
• Description: Rabbit Polyclonal
• Gene: CSTB
• Conjugate: Unconjugated

Product images

ICC/IF image of ab53725 stained MCF-7 cells. The cells were 100% methanol fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53725, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Ab53725 staining Human normal placenta. Staining is localized to cytoplasmic and nuclear compartments.Left panel: with primary antibody at 1ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

All lanes : Anti-Stefin B antibody (ab53725) at 1 µg/mlLane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : COLO 205 (Human colon adenocarcinoma cell line) Whole Cell Lysate Lane 4 : HCT 116 (Human Colorectal Carcinoma) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Product References

Cystatin B is a progression marker of human epithelial ovarian tumors mediated by - Cystatin B is a progression marker of human epithelial ovarian tumors mediated by

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Gain in toxic function of stefin B EPM1 mutants aggregates: correlation between - Gain in toxic function of stefin B EPM1 mutants aggregates: correlation between

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Characterization of PTZ-induced seizure susceptibility in a down syndrome mouse - Characterization of PTZ-induced seizure susceptibility in a down syndrome mouse

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Nuclear cathepsin F regulates activation markers in rat hepatic stellate cells. - Nuclear cathepsin F regulates activation markers in rat hepatic stellate cells.

Maubach G, Lim MC, Zhuo L. Mol Biol Cell. 2008 Oct;19(10):4238-48.