Lane 1 : Anti-Stefin B antibody [RJMW-2E7] (ab62718) at 2 µg/mlLane 2 : Anti-Stefin B antibody [RJMW-2E7] (ab62718) at 1 µg/mlLane 3 : Anti-Stefin B antibody [RJMW-2E7] (ab62718) at 0.5 µg/mlLane 4 : Anti-Stefin B antibody [RJMW-2E7] (ab62718) at 0.25 µg/mlLane 5 : Anti-Stefin B antibody [RJMW-2E7] (ab62718) at 0.12 µg/mlLane 1 : Jurkat total cell extractLane 2 : Jurkat total cell extractLane 3 : Jurkat total cell extractLane 4 : Jurkat total cell extractLane 5 : Jurkat total cell extractSecondaryGoat Anti-Mouse IgG, Peroxidase conjugate developed using the ECL technique