Anti-STIP1 antibody
| Name | Anti-STIP1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab56873 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB IHC-P IP FC |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-544 of Human STIP1 |
| Description | Mouse Monoclonal |
| Gene | STIP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
STIP1 antibody (ab56873) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human testis.
![Overlay histogram showing HeLa cells stained with ab56873 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56873, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/6173_STIP1-Primary-antibodies-ab56873-1.jpg)
Overlay histogram showing HeLa cells stained with ab56873 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56873, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Identification of direct tyrosine kinase substrates based on protein kinase - Identification of direct tyrosine kinase substrates based on protein kinase
Xue L, Geahlen RL, Tao WA. Mol Cell Proteomics. 2013 Oct;12(10):2969-80.
RNAi knockdown of Hop (Hsp70/Hsp90 organising protein) decreases invasion via - RNAi knockdown of Hop (Hsp70/Hsp90 organising protein) decreases invasion via
Walsh N, Larkin A, Swan N, Conlon K, Dowling P, McDermott R, Clynes M. Cancer Lett. 2011 Jul 28;306(2):180-9.