Anti-STK39 antibody
| Name | Anti-STK39 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab71825 |
| Prices | $359.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide from the central region of human STK39 conjugated to KLH |
| Description | Rabbit Polyclonal |
| Gene | STK39 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-STK39 antibody (ab71825) at 1/100 dilution + mouse liver tissue lysate at 12.5 µgSecondaryHRP-anti-rabbit
ab71825 at 1/50 dilution, staining STK39 in human hepatocarcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.
ICC/IF image of ab71825 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71825, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.