2 µg of ab128044 was added to aliquots of cleared cell lysates from transfected Human cell lines and incubated for 2 hr at 4°C. Protein G Dynabeads were then added, and lysates were incubated for an additional 2 hr at 4°C to capture the IgG-protein complex. Anti-V5 antibody was used in parallel as a positive control. As a negative control, primary antibody (ab128044) was excluded. Lane 1: Cell lysate control Lane 2: Cell lysate with ab128044 Lane 3: Cell lysate without ab128044 Lane 4: V5 tag control