All lanes : Anti-STOM antibody [EPR10421] (ab166623) at 1/1000 dilutionLane 1 : Human fetal heart lysatesLane 2 : HeLa lysatesLane 3 : HepG2 lysatesLane 4 : Neuro-2a lysatesLane 5 : Raw264.7 lysatesLysates/proteins at 10 µg per lane.
Immunofluorescence analysis of HeLa cells labeling STOM with ab166623 at 1/100 dilution.
Flow cytometric analysis of permeabilized Neuro-2a cells using ab166623 (red) at 1/10 dilution or a rabbit IgG (negative) (green).
Immunoprecipitation of STOM in pellet from Human fetal heart lysate using ab166623 at 1/10 dilution, followed by WB analysis of STOM.