All lanes : Anti-STOM antibody - C-terminal (ab190498) at 0.4 µg/mlLane 1 : HeLa cell lysate using NETN bufferLane 2 : HeLa cell lysate using RIPA bufferLane 3 : 293T cell lysate using NETN bufferLane 4 : Jurkat cell lysate using NETN bufferLysates/proteins at 50 µg per lane.developed using the ECL technique
Immunoprecipitation analysis of HeLa cell lysate (using RIPA buffer, 1 mg per IP reaction; 20% of IP loaded) labeling STOM using ab190498 at 6 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated STOM, ab190498 was used at 1 μg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.