All lanes : Anti-SULT2A1 antibody [EPR16096] (ab194113) at 1/10000 dilutionLane 1 : HepG2 cell lysateLane 2 : Human fetal liver lysateLane 3 : Human adrenal gland lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-SULT2A1 antibody [EPR16096] (ab194113) at 1/2000 dilutionLane 1 : Mouse spleen lysateLane 2 : Rat kidney lysateLane 3 : Rat liver lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human adrenal tissue labeling SULT2A1 with ab194113 at 1/4000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labeling SULT2A1 with ab194113 at 1/50 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).
Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 cells labeling SULT2A1 with ab194113 at 1/120 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.