Anti-SUNC1 antibody [EPR12910] (ab181063) at 1/1000 dilution + LnCap cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-SUNC1 antibody [EPR12910] (ab181063) at 1/1000 dilution + HepG2 cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of paraformaldehyde-fixed HepG2 cells labeling SUNC1 with ab181063 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) was used as the secondary at 1/200 dilution.
Flow Cytometric analysis of HepG2 cells (2% paraformaldehyde-fixed) labeling SUNC1 with ab181063 at 1/110 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.