Anti-Superoxide Dismutase 1 antibody

• Supplier: Abcam
• Catalog: ab16831
• Prices: $401.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF ICC/IF IHC-P IP IHC-F
• Species Reactivities: Mouse, Rat, Human, Monkey
• Antigen: Recombinant full length protein (Human) purified from E Coli
• Description: Rabbit Polyclonal
• Gene: SOD1
• Conjugate: Unconjugated

Product images

All lanes : Anti-Superoxide Dismutase 1 antibody (ab16831) at 1/2000 dilutionLane 1 : 40ug mouse liver homogenateLane 2 : 20ug mouse liver homogenateLane 3 : 5ug mouse liver homogenateSecondaryHRP conjugated donkey anti-rabbit IgGdeveloped using the ECL techniquePerformed under reducing conditions.

Ab16831 staining human normal placenta tissue. Staining is localised to cytoplasm.Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

ab16831 staining superoxide dismutase in mouse liver tissue by immunohistochemistry (frozen sections). Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 2% BSA for 10 minutes at 21°C. The primary antibody was diluted 1/200 and incubated with the sample for 9 hours at 4°C. Alexa fluor® 488 goat polyclonal to rabbit Ig, diluted 1/200, was used as the secondary.See Abreview

Product References

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