All lanes : Anti-Superoxide Dismutase 1 antibody (ab45777) at 0.5 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLane 8 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human Superoxide Dismutase 1 peptide (ab46993) at 1 µgLane 9 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899) with Human Superoxide Dismutase 1 peptide (ab46993) at 1 µg/mlLane 10 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909) with Human Superoxide Dismutase 1
ICC/IF image of ab45777 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab45777, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
All lanes : Anti-Superoxide Dismutase 1 antibody (ab45777) at 1/1000 dilutionLane 1 : Whole tissue lysate prepared from human breast cancer tissueLane 2 : Whole tissue lysate prepared from human breast cancer tissueLysates/proteins at 8 µg per lane.SecondaryHRP conjugated goat polyclonal to rabbit IgG at 1/1000 dilutionPerformed under reducing conditions.
ab45777 (1/2000) staining SOD1 in paraffin-embedded human breast carcinoma sections. Tissues underwent formaldehyde fixation, heat-mediated antigen retrieval and peroxidase-blocking. Secondary antibody was a goat anti-rabbit IgG conjugated to HRP. For further experimental details please refer to Abreview.See Abreview