All lanes : Anti-Superoxide Dismutase 3 antibody (ab90258) at 1 µg/mlLane 1 : Blood Vessel: artery (Human) Membrane Lysate - adult normal tissue (ab28989)Lane 2 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 3 : Lung (Human) Tissue LysateLane 4 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.