IHC image of ab28442 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28442, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![All lanes : Anti-Superoxide Dismutase 3 antibody [1H12] (ab28442)Lane 1 : Bosc23 cell lysateLane 2 : Bosc23 cell lysate transfected with Myc-His-SOD3Lane 3 : Bosc23 cell lysate transfected with Myc-His-Gpx3](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/6928_Ab28442_1.JPG)
All lanes : Anti-Superoxide Dismutase 3 antibody [1H12] (ab28442)Lane 1 : Bosc23 cell lysateLane 2 : Bosc23 cell lysate transfected with Myc-His-SOD3Lane 3 : Bosc23 cell lysate transfected with Myc-His-Gpx3