Anti-Surf1 antibody [21H2BG4]
| Name | Anti-Surf1 antibody [21H2BG4] |
|---|---|
| Supplier | Abcam |
| Catalog | ab110256 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 21H2BG4 |
| Applications | WB FC |
| Species Reactivities | Human |
| Antigen | Recombinant Human Surf1 |
| Description | Mouse Monoclonal |
| Gene | SURF1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HepG2 cells stained with ab110256 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110256, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/7009_ab110256-2-ab110256FC.jpg)
Overlay histogram showing HepG2 cells stained with ab110256 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110256, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
SURF1 deficiency causes demyelinating Charcot-Marie-Tooth disease. - SURF1 deficiency causes demyelinating Charcot-Marie-Tooth disease.
Echaniz-Laguna A, Ghezzi D, Chassagne M, Mayencon M, Padet S, Melchionda L, Rouvet I, Lannes B, Bozon D, Latour P, Zeviani M, Mousson de Camaret B. Neurology. 2013 Oct 22;81(17):1523-30.
Beneficial effects of resveratrol on respiratory chain defects in patients' - Beneficial effects of resveratrol on respiratory chain defects in patients'
Lopes Costa A, Le Bachelier C, Mathieu L, Rotig A, Boneh A, De Lonlay P, Tarnopolsky MA, Thorburn DR, Bastin J, Djouadi F. Hum Mol Genet. 2014 Apr 15;23(8):2106-19.
Activation of peroxisome proliferator-activated receptor pathway stimulates the - Activation of peroxisome proliferator-activated receptor pathway stimulates the
Bastin J, Aubey F, Rotig A, Munnich A, Djouadi F. J Clin Endocrinol Metab. 2008 Apr;93(4):1433-41.
Increased longevity and refractoriness to Ca(2+)-dependent neurodegeneration in - Increased longevity and refractoriness to Ca(2+)-dependent neurodegeneration in
Dell'agnello C, Leo S, Agostino A, Szabadkai G, Tiveron C, Zulian A, Prelle A, Roubertoux P, Rizzuto R, Zeviani M. Hum Mol Genet. 2007 Feb 15;16(4):431-44. Epub 2007 Jan 8.