Anti-SURF4 antibody [EPR16371]

• Supplier: Abcam
• Catalog: ab192611
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16371
• Applications: WB ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SURF4 aa 100-200
• Description: Rabbit Monoclonal
• Gene: SURF4
• Conjugate: Unconjugated

Product images

Flow cytometric analysis of Jurkat cells (paraformaldehyde-fixed, 2%) labeling SURF4 with ab192611 at 1/20 dilution (red) or a rabbit IgG (negative) (blue), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Immunofluorescent analysis of HepG2 cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling SURF4 with ab192611 at 1/50 dilution (2μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).

All lanes : Anti-SURF4 antibody [EPR16371] (ab192611) at 1/1000 dilutionLane 1 : C6 lysateLane 2 : RAW 264.7 lysateLane 3 : PC12 lysateLane 4 : NIH 3T3 lysateLane 5 : Mouse brain lysateLane 6 : Mouse heart lysateLane 7 : Mouse kidney lysateLane 8 : Mouse spleen lysateLane 9 : Rat brain lysateLane 10 : Rat heart lysateLane 11 : Rat kidney lysateLane 12 : Rat spleen lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Anti-SURF4 antibody [EPR16371] (ab192611) at 1/1000 dilution + Human fetal kidney lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Lane 1 : Lane 2 : Lane 3 : Lane 1 : HepG2 cell lysate with Anti-SURF4 antibody [EPR16371] (ab192611)Lane 2 : HeLa cell lysate with Anti-SURF4 antibody [EPR16371] (ab192611)Lane 3 : Jurkat cell lysate with Anti-SURF4 antibody [EPR16371] (ab192611)Lysates/proteins at 20 µg per lane.Blocking peptides at 1/10000 dilution per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution