Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling Survivin with ab175809 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature. From left to right - Survivin, CyclinB, DAPI, Merge.
Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling Survivin with ab175809 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature. Left - Survivin, Middle - DAPI, Right - Merge.
Flow Cytometry analysis of HeLa cells labelling Survivin with ab175809 at 1:400. Cells were fixed with 1% PFA. Cells were incuabted with the primary antibody overnight at 4°C.
Immunoprecipitation analysis of U2OS cells labelling Survivin with ab175809. Protein A beads were crosslinked to the serum.
All lanes : Anti-Survivin antibody (ab175809)Lane 1 : Control siLane 2 : Survivin siLane 3 : NIH 3T3 cell lysate at 40 µg