Anti-Survivin DEx3 antibody
| Name | Anti-Survivin DEx3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab3731 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide of Human Survivin DEx3, derived from amino acids 50-150 |
| Blocking Peptide | Human Survivin DEx3 peptide |
| Description | Rabbit Polyclonal |
| Gene | BIRC5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab3731 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3731, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Survivin transcript variant 2 drives angiogenesis and malignant progression in - Survivin transcript variant 2 drives angiogenesis and malignant progression in
Doucette T, Latha K, Yang Y, Fuller GN, Rao A, Rao G. Neuro Oncol. 2014 Sep;16(9):1220-8.
Association of p53 gene alterations with the expression of antiapoptotic survivin - Association of p53 gene alterations with the expression of antiapoptotic survivin
Vegran F, Boidot R, Oudin C, Defrain C, Rebucci M, Lizard-Nacol S. Oncogene. 2007 Jan 11;26(2):290-7. Epub 2006 Jul 17.
Molecular analysis of survivin isoforms: evidence that alternatively spliced - Molecular analysis of survivin isoforms: evidence that alternatively spliced
Noton EA, Colnaghi R, Tate S, Starck C, Carvalho A, Ko Ferrigno P, Wheatley SP. J Biol Chem. 2006 Jan 13;281(2):1286-95. Epub 2005 Nov 16.
Survivin splice variants regulate the balance between proliferation and cell - Survivin splice variants regulate the balance between proliferation and cell
Caldas H, Jiang Y, Holloway MP, Fangusaro J, Mahotka C, Conway EM, Altura RA. Oncogene. 2005 Mar 17;24(12):1994-2007.
Survivin splice variants regulate the balance between proliferation and cell - Survivin splice variants regulate the balance between proliferation and cell
Caldas H, Jiang Y, Holloway MP, Fangusaro J, Mahotka C, Conway EM, Altura RA. Oncogene. 2005 Mar 17;24(12):1994-2007.