Immunofluorescent analysis of 4% paraformaldehyde-fixed A431cells labeling SVH with ab188573 at 1/50 followed by Goat anti rabbit IgG (Alexa Fluor®488) and DAPI staining.
All lanes : Anti-SVH antibody [EPR13763] - C-terminal (ab188573) at 1/1000 dilutionLane 1 : SKBR-3 lysateLane 2 : A431 lysateLane 3 : HepG2 lysateLane 4 : U87-MG lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-SVH antibody [EPR13763] - C-terminal (ab188573) at 1/1000 dilutionLane 1 : C6 lysateLane 2 : RAW264.7 lysateLane 3 : PC-12 lysateLane 4 : NIH3T3 lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Western blot analysis of SVH in immunoprecipitation pellets from U87-MG lysate. ab188573 used at 1/50 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1500 dilution.