Anti-Synaptophysin antibody

Name	Anti-Synaptophysin antibody
Supplier	Abcam
Catalog	ab32594
Prices	$395.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Applications	WB IHC-F ICC/IF ICC/IF IHC-F
Species Reactivities	Mouse, Rat, Sheep, Bovine, Zebrafish, Fish, Human
Antigen	Synthetic peptide: GPGGYGPQDSYGPQGGYQPD , corresponding to amino acids 253-272 of Rat Synaptophysin Run BLAST with Run BLAST with
Description	Rabbit Polyclonal
Gene	SYP
Conjugate	Unconjugated
Supplier Page	Shop

Product images

ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.

Anti-Synaptophysin antibody (ab32594) at 1/5000 dilution + Rat brain

Anti-Synaptophysin antibody (ab32594) at 1/1000 dilution + Apteronotus leptorhynchus brain tissue lysate at 50 µgSecondaryAlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG at 1/1000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Immunohistochemical analysis of Apteronotus leptorhynchus brain tissue, staining Synaptophysin with ab32594.Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 3% sheep serum, 1% BSA and 1% teleostean gelatine in TBS for 1 hour at 24°. Samples were incubated with primary antibody (1/100 in blocking solution) for 18 hours at 4°C. An AlexaFluor®546-conjugated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody. See Abreview

Product References

Ghrelin triggers the synaptic incorporation of AMPA receptors in the hippocampus. - Ghrelin triggers the synaptic incorporation of AMPA receptors in the hippocampus.

Ribeiro LF, Catarino T, Santos SD, Benoist M, van Leeuwen JF, Esteban JA, Carvalho AL. Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):E149-58. doi: