Anti-Synaptophysin antibody [SY38]
| Name | Anti-Synaptophysin antibody [SY38] |
|---|---|
| Supplier | Abcam |
| Catalog | ab8049 |
| Prices | $401.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | SY38 |
| Applications | WB FC ICC/IF ICC/IF IHC-F IHC-P |
| Species Reactivities | Mouse, Rat, Hamster, Bovine, Human |
| Antigen | Presynaptic vesicles |
| Description | Mouse Monoclonal |
| Gene | SYP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab8049 staining Synaptophysin in mouse hippocampus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 1 hour at 24°C. Samples were incubated with primary antibody (1/100 in BSA) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.See Abreview
The pictures show immunohistochemical staining of normal mucosa and neuroendocrine tumour of the small intestine. The antibody (ab8049) stains positive in a 1:10 dilution in nerve cells of the colon as well as some cells in normal colon mucosa and some neuroendocrine tumors of the digestive tract. Staining protocol: Mouse monoclonal to Synaptophysin (ab8049), diluted 1:10 in PBS plus BSA, 60 min at room temperature, secondary antibody: HRP solution with anti mouse polyclonal antibodies, 30min. DAB staining 10 min; Hematoxylin 30 sec.These images were kindly supplied as part of the review submitted by Karin Birkenkamp-Demtroeder.
![All lanes : Anti-Synaptophysin antibody [SY38] (ab8049) at 1/500 dilutionLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Brain (Mouse) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed at 1/3000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/7469_Synaptophysin-Primary-antibodies-ab8049-2.jpg)
All lanes : Anti-Synaptophysin antibody [SY38] (ab8049) at 1/500 dilutionLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Brain (Mouse) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed at 1/3000 dilution
![Overlay histogram showing PC12 cells stained with ab8049 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8049, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/7470_Synaptophysin-Primary-antibodies-ab8049-5.jpg)
Overlay histogram showing PC12 cells stained with ab8049 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8049, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.
Product References
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