Anti-Synaptophysin antibody [YE269]

Name	Anti-Synaptophysin antibody [YE269]
Supplier	Abcam
Catalog	ab32127
Prices	$99.00, $401.00
Sizes	10 µl, 100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	YE269
Applications	WB ICC/IF ICC/IF IHC-F IHC-P
Species Reactivities	Mouse, Rat, Human, Bovine
Antigen	A synthetic peptide corresponding to residues in C terminus (cytoplasmic domain) of human Synaptophysin
Blocking Peptide	Synaptophysin peptide
Description	Rabbit Monoclonal
Gene	SYP
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-Synaptophysin antibody [YE269] (ab32127) at 1/100000 dilution (purified)Lane 1 : Mouse brainLane 2 : Rat brainSecondaryHRP goat anti-rabbit at 1/1000 dilution

Immunofluorescent staining of PC-12 cells (fixed in 4% PFA, permeabilized with 0.1% Triton X 100) using purified ab32127 at a dilution of 1/50. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/500 and the cells were counter stained with DAPI. The negative control is shown in the bottom right hand panel - for the negative control, Alex Fluor® 594 goat anti-mouse was used at a dilution of 1/500.

Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab32127 at a dilution of 1/400. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained wirh hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunohistochemical staining of paraffin embedded mouse cerebral cortex with purified ab32127 at a dilution of 1/400. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained wirh hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunohistochemical staining of paraffin embedded human pancreas with purified ab32127 at a dilution of 1/400. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained wirh hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Anti-Synaptophysin antibody [YE269] (ab32127) at 1/100000 dilution (purified) + Human fetal brain at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-Synaptophysin antibody [YE269] (ab32127) at 1/10000 dilution (unpurified) + PC12 cell lysate

Unpurified ab32127 showing positive staining in Medulloblastoma tissue.

Unpurified ab32127 showing positive staining in Lung neuroendocrine tumor tissue.

Product References

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