Anti-Synaptotagmin 4 antibody
| Name | Anti-Synaptotagmin 4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab57473 |
| Prices | $380.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG3 |
| Applications | WB ICC/IF ICC/IF IHC-F |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: MAPITTSREE FDEIPTVVGI FSAFGLVFTV SLFAWICCQR KSSKSNKTPP YKFVHVLKGV DIYPENLNSK KKFGADDKNE VKNKPAVPKN SLHLDLEKR, corresponding to amino acids 1-100 of Human Synaptotagmin 4 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | SYT4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab57473 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57473, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Synaptotagmin IV determines the linear Ca2+ dependence of vesicle fusion at - Synaptotagmin IV determines the linear Ca2+ dependence of vesicle fusion at
Johnson SL, Franz C, Kuhn S, Furness DN, Ruttiger L, Munkner S, Rivolta MN, Seward EP, Herschman HR, Engel J, Knipper M, Marcotti W. Nat Neurosci. 2010 Jan;13(1):45-52.
Synaptotagmin-IV modulates synaptic function and long-term potentiation by - Synaptotagmin-IV modulates synaptic function and long-term potentiation by
Dean C, Liu H, Dunning FM, Chang PY, Jackson MB, Chapman ER. Nat Neurosci. 2009 Jun;12(6):767-76.