Anti-Syndecan 4 antibody

• Supplier: Abcam
• Catalog: ab24511
• Prices: $391.00
• Sizes: 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-P IHC-F IP WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide surrounding amino acid 184 of human Syndecan 4 (Intracellular domain)
• Blocking Peptide: Syndecan 4 peptide
• Description: Rabbit Polyclonal
• Gene: SDC4
• Conjugate: Unconjugated

Product images

Anti-Syndecan 4 antibody (ab24511) + Jurkat Cell lysate

ab24511 (1µg/ml) staining Syndecan 4 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cellular membrane compartment in the subset of intestinal glands.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

ICC/IF image of ab24511 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24511, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemical analysis of mature rat intervertebral disc tissue, staining Syndecan 4 with ab24511. Tissue was fixed in 4% paraformaldehyde before incubating with primary antibody (1/200) overnight at 4°C. An AlexaFluor®488-conjugated anti-rabbit IgG (1/200) was used as the secondary antibody.

Product References

Angiopoietin-2 secretion by endothelial cell exosomes: regulation by the - Angiopoietin-2 secretion by endothelial cell exosomes: regulation by the

Ju R, Zhuang ZW, Zhang J, Lanahan AA, Kyriakides T, Sessa WC, Simons M. J Biol Chem. 2014 Jan 3;289(1):510-9.

Heparan sulfate regulates hair follicle and sebaceous gland morphogenesis and - Heparan sulfate regulates hair follicle and sebaceous gland morphogenesis and

Coulson-Thomas VJ, Gesteira TF, Esko J, Kao W. J Biol Chem. 2014 Sep 5;289(36):25211-26.

The syndecan-4/protein kinase Calpha pathway mediates prostaglandin E2-induced - The syndecan-4/protein kinase Calpha pathway mediates prostaglandin E2-induced

Corti F, Finetti F, Ziche M, Simons M. J Biol Chem. 2013 May 3;288(18):12712-21.

Heparanase-induced GEF-H1 signaling regulates the cytoskeletal dynamics of brain - Heparanase-induced GEF-H1 signaling regulates the cytoskeletal dynamics of brain

Ridgway LD, Wetzel MD, Ngo JA, Erdreich-Epstein A, Marchetti D. Mol Cancer Res. 2012 Jun;10(6):689-702.

Prolyl hydroxylase 3 (PHD3) modulates catabolic effects of tumor necrosis - Prolyl hydroxylase 3 (PHD3) modulates catabolic effects of tumor necrosis

Fujita N, Gogate SS, Chiba K, Toyama Y, Shapiro IM, Risbud MV. J Biol Chem. 2012 Nov 16;287(47):39942-53.

Heparanase Modulates Shh and Wnt3a Signaling in Human Medulloblastoma Cells. - Heparanase Modulates Shh and Wnt3a Signaling in Human Medulloblastoma Cells.

Ridgway LD, Wetzel MD, Marchetti D. Exp Ther Med. 2011 Mar 1;2(2):229-238.

TNF-alpha and IL-1beta promote a disintegrin-like and metalloprotease with - TNF-alpha and IL-1beta promote a disintegrin-like and metalloprotease with

Wang J, Markova D, Anderson DG, Zheng Z, Shapiro IM, Risbud MV. J Biol Chem. 2011 Nov 18;286(46):39738-49.

Virally-induced upregulation of heparan sulfate on B cells via the action of type - Virally-induced upregulation of heparan sulfate on B cells via the action of type

Jarousse N, Trujillo DL, Wilcox-Adelman S, Coscoy L. J Immunol. 2011 Dec 1;187(11):5540-7.

Modulation of GEF-H1 induced signaling by heparanase in brain metastatic melanoma - Modulation of GEF-H1 induced signaling by heparanase in brain metastatic melanoma

Ridgway LD, Wetzel MD, Marchetti D. J Cell Biochem. 2010 Dec 1;111(5):1299-309.

BAFF-R promotes cell proliferation and survival through interaction with IKKbeta - BAFF-R promotes cell proliferation and survival through interaction with IKKbeta

Fu L, Lin-Lee YC, Pham LV, Tamayo AT, Yoshimura LC, Ford RJ. Blood. 2009 May 7;113(19):4627-36.