Cre Recombinase (D3U7F) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 12830
• Prices: $246.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D3U7F
• Applications: WB ICC/IF FC
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly103 of bacteriophage-P1 Cre recombinase protein.
• Description: Rabbit Monoclonal

Product images

Western blot analysis of extracts from 293T cells, mock-transfected (-) or transfected with a construct expressing Cre recombinase (+), using Cre Recombinase (D3U7F) Rabbit mAb (upper) and β-Actin (13E5) Rabbit mAb #4970 (lower).

Confocal immunofluorescent analysis of ER-Cre-MEF cells, untreated (left) or treated with 4OH-Tamoxifen (200 ng/ml; center), and Cre-transfected 293 cells (right), using Cre Recombinase (D3U7F) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye). The ER-Cre-MEF cells were kindly provided by Dr. Mark Bedford (M.D. Anderson Cancer Center, TX).

Flow cytometric analysis of COS-7 cells, untransfected (blue) or transfected with Cre recombinase (green), using Cre Recombinase (D3U7F) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) #4414 was used as a secondary antibody.