Anti-Syntaxin 2 antibody
| Name | Anti-Syntaxin 2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab12369 |
| Prices | $370.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP ICC/IF |
| Species Reactivities | Mouse, Rat, Sheep, Rabbit, Hamster, Bovine, Human, Pig, Xenopus, Drosophila, Monkey |
| Antigen | Synthetic peptide: MRDRLPDLTACRKSDDGDN conjugated to KLH, corresponding to amino acids 1-19 of Rat Syntaxin 2 |
| Description | Rabbit Polyclonal |
| Gene | STX2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Syntaxin 2 antibody (ab12369) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 4 : Skin (Human) Tissue Lysate - adult normal tissue (ab30166)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab12369 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12369, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Deficiency in the omega-3 fatty acid pathway results in failure of acrosome - Deficiency in the omega-3 fatty acid pathway results in failure of acrosome
Roqueta-Rivera M, Abbott TL, Sivaguru M, Hess RA, Nakamura MT. Biol Reprod. 2011 Oct;85(4):721-32.
SNARE proteins mediate fusion between cytosolic lipid droplets and are implicated - SNARE proteins mediate fusion between cytosolic lipid droplets and are implicated
Bostrom P, Andersson L, Rutberg M, Perman J, Lidberg U, Johansson BR, Fernandez-Rodriguez J, Ericson J, Nilsson T, Boren J, Olofsson SO. Nat Cell Biol. 2007 Nov;9(11):1286-93. Epub 2007 Oct 7.
Characterization of immunoisolated human gastric parietal cells tubulovesicles: - Characterization of immunoisolated human gastric parietal cells tubulovesicles:
Lapierre LA, Avant KM, Caldwell CM, Ham AJ, Hill S, Williams JA, Smolka AJ, Goldenring JR. Am J Physiol Gastrointest Liver Physiol. 2007 May;292(5):G1249-62. Epub 2007 Jan
Localization of SNAREs, NSF and Caveolin 1 in human spermatozoa: relationship - Localization of SNAREs, NSF and Caveolin 1 in human spermatozoa: relationship
Sousa AP, Gomes-Santos CS, Ramalho-Santos J. Arch Androl. 2006 Sep-Oct;52(5):347-53.