Anti-Syntaxin 6 antibody [3D10]
| Name | Anti-Syntaxin 6 antibody [3D10] |
|---|---|
| Supplier | Abcam |
| Catalog | ab12370 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 3D10 |
| Applications | FC WB IP ICC/IF |
| Species Reactivities | Mouse, Rat, Hamster, Human |
| Antigen | Recombinant rat syntaxin 6 fusion protein |
| Description | Mouse Monoclonal |
| Gene | STX6 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab12370 at 1/200 staining a mouse macrophage cell line by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 24 hours. A FITC conjugated donkey anti-mouse antibody was used as the secondary.See Abreview
![All lanes : Anti-Syntaxin 6 antibody [3D10] (ab12370) at 1 µg/mlLane 1 : Brain (Mouse) Tissue LysateLane 2 : Brain (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/7741_Syntaxin-6-Primary-antibodies-ab12370-2.jpg)
All lanes : Anti-Syntaxin 6 antibody [3D10] (ab12370) at 1 µg/mlLane 1 : Brain (Mouse) Tissue LysateLane 2 : Brain (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Syntaxin 6 was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 10µg of Mouse monoclonal to Syntaxin 6 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab12370.Secondary: Protein G-HRP at 1/500 dilution.Band: 25kDa:Syntaxin 6.
 used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/7744_Syntaxin-6-Primary-antibodies-ab12370-4.jpg)
Overlay histogram showing PC12 cells stained with ab12370 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12370, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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