Anti-Syntaxin antibody [STX01 (HPC-1)]
| Name | Anti-Syntaxin antibody [STX01 (HPC-1)] |
|---|---|
| Supplier | Abcam |
| Catalog | ab3265 |
| Prices | $384.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | STX01 (HPC-1) |
| Applications | WB IP ICC/IF ICC/IF |
| Species Reactivities | Rat, Human |
| Antigen | amino acids 2-335 of Rat Syntaxin |
| Description | Mouse Monoclonal |
| Gene | STX1B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188)Lane 3 : Spinal Cord (Rat) Tissue LysateLane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/7771_Syntaxin-Primary-antibodies-ab3265-2.jpg)
All lanes : Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188)Lane 3 : Spinal Cord (Rat) Tissue LysateLane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab3265 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3265, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25. - LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25.
Yun HJ, Park J, Ho DH, Kim H, Kim CH, Oh H, Ga I, Seo H, Chang S, Son I, Seol W. Exp Mol Med. 2013 Aug 16;45:e36.
Amacrine cell subtypes differ in their intrinsic neurite growth capacity. - Amacrine cell subtypes differ in their intrinsic neurite growth capacity.
Kunzevitzky NJ, Willeford KT, Feuer WJ, Almeida MV, Goldberg JL. Invest Ophthalmol Vis Sci. 2013 Nov 15;54(12):7603-13. doi:
Foxn4 is required for retinal ganglion cell distal axon patterning. - Foxn4 is required for retinal ganglion cell distal axon patterning.
Kunzevitzky NJ, Almeida MV, Duan Y, Li S, Xiang M, Goldberg JL. Mol Cell Neurosci. 2011 Apr;46(4):731-41.
VAMP-2, SNAP-25A/B and syntaxin-1 in glutamatergic and GABAergic synapses of the - VAMP-2, SNAP-25A/B and syntaxin-1 in glutamatergic and GABAergic synapses of the
Benagiano V, Lorusso L, Flace P, Girolamo F, Rizzi A, Bosco L, Cagiano R, Nico B, Ribatti D, Ambrosi G. BMC Neurosci. 2011 Nov 17;12:118.
Amacrine cell gene expression and survival signaling: differences from - Amacrine cell gene expression and survival signaling: differences from
Kunzevitzky NJ, Almeida MV, Goldberg JL. Invest Ophthalmol Vis Sci. 2010 Jul;51(7):3800-12.