All lanes : Anti-SYPL1 antibody [EPR14317] (ab184176) at 1/5000 dilutionLane 1 : Human fetal lungLane 2 : Human fetal liverLysates/proteins at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
All lanes : Anti-SYPL1 antibody [EPR14317] (ab184176) at 1/1000 dilutionLane 1 : Human fetal kidney lysateLane 2 : Human adipose tissue lysateLysates/proteins at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Flow cytometric analysis of HepG2 cells (paraformaldehyde-fixed, 2%) labeling SYPL1 with ab184176 at 1/130 dilution (red) or a rabbit IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SYPL1 with ab184176 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SYPL1 with ab184176 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.