Anti-T Plastin antibody

• Supplier: Abcam
• Catalog: ab45769
• Prices: $379.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ELISA IHC-P
• Species Reactivities: Human
• Antigen: Synthetic peptide corresponding to the N-terminal of human T Plastin
• Description: Rabbit Polyclonal
• Gene: PLS3
• Conjugate: Unconjugated

Product images

All lanes : Anti-T Plastin antibody (ab45769) at 1/1000 dilutionLane 1 : Bacterial extract expressing T-Plastin at 1 µgLane 2 : Bacterial extract expressing L-Plastin at 1 µgLane 3 : Bacterial extract expressing I-Plastin at 1 µgLane 4 : Sezary cell extract at 10 µgLane 5 : Control T-Helper cells at 10 µg

IHC image of ab45769 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45769, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

Regulation of T-plastin expression by promoter hypomethylation in primary - Regulation of T-plastin expression by promoter hypomethylation in primary

Jones CL, Ferreira S, McKenzie RC, Tosi I, Caesar JA, Bagot M, Whittaker SJ, Mitchell TJ. J Invest Dermatol. 2012 Aug;132(8):2042-9.

Proteomic similarities in steroid responsiveness in normal and glaucomatous - Proteomic similarities in steroid responsiveness in normal and glaucomatous

Bollinger KE, Crabb JS, Yuan X, Putliwala T, Clark AF, Crabb JW. Mol Vis. 2012;18:2001-11. Epub 2012 Jul 20.