Overlay histogram showing HeLa cells stained with ab76412 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76412, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![Anti-TAB1 antibody [EPR635Y] (ab76412) at 1/2000 dilution + MCF-7 cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/7971_ab76412.jpg)
Anti-TAB1 antibody [EPR635Y] (ab76412) at 1/2000 dilution + MCF-7 cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Immunohistochemical staining of TAB1 in paraffin embedded human tonsils using ab76412 at a 1/100 dilution.