IHC image of ab65006 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab65006, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-TACD2 antibody (ab65006) at 1/500 dilutionLane 1 : Extracts from A549 cellsLane 2 : Extracts from NIH-3T3 cellsLane 3 : Extracts from NIH-3T3 cells with immunising peptideLysates/proteins at 5 µg per lane.