Anti-TACD2 antibody [162-46.2]
| Name | Anti-TACD2 antibody [162-46.2] |
|---|---|
| Supplier | Abcam |
| Catalog | ab79976 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 162-46.2 |
| Applications | IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | Human choriocarcinoma cell line BeWo |
| Description | Mouse Monoclonal |
| Gene | TACSTD2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab79976 staining TACD2 in human 10 week placenta by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Anti-mouse peroxidase IgG used as secondary antibody. Note staining of cytotrophoblast (CT) and syncytiotrophoblast (ST).
ICC/IF image of ab79976 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79976, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing MCF7 cells stained with ab79976 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79976, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed MCF7 cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/8035_TACD2-Primary-antibodies-ab79976-5.jpg)
Overlay histogram showing MCF7 cells stained with ab79976 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79976, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed MCF7 cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
Interactome analysis reveals ezrin can adopt multiple conformational states. - Interactome analysis reveals ezrin can adopt multiple conformational states.
Viswanatha R, Wayt J, Ohouo PY, Smolka MB, Bretscher A. J Biol Chem. 2013 Dec 6;288(49):35437-51.
Human trophoblast cell-surface antigens defined by monoclonal antibodies. - Human trophoblast cell-surface antigens defined by monoclonal antibodies.
Lipinski M, Parks DR, Rouse RV, Herzenberg LA. Proc Natl Acad Sci U S A. 1981 Aug;78(8):5147-50.