Anti-TAPA1 antibody [M38]
| Name | Anti-TAPA1 antibody [M38] |
|---|---|
| Supplier | Abcam |
| Catalog | ab79559 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | M38 |
| Applications | WB IP IHC-P FA ICC/IF FC |
| Species Reactivities | Rabbit, Cat, Human |
| Antigen | MOLT4 (human T-ALL cell line) |
| Description | Mouse Monoclonal |
| Gene | CD81 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of TAPA1 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79559, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Overlay histogram showing MOLT4 cells stained with ab79559 (red line). The cells were fixed with 4% paraformaldehyde and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79559, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MOLT4 cells fixed with methanol (5 min) used under the same conditions.Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/8411_TAPA1-Primary-antibodies-ab79559-1.jpg)
Overlay histogram showing MOLT4 cells stained with ab79559 (red line). The cells were fixed with 4% paraformaldehyde and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79559, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MOLT4 cells fixed with methanol (5 min) used under the same conditions.Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
Selective enrichment of tetraspan proteins on the internal vesicles of - Selective enrichment of tetraspan proteins on the internal vesicles of
Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ. J Biol Chem. 1998 Aug 7;273(32):20121-7.
C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to - C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to
Imai T, Yoshie O. J Immunol. 1993 Dec 1;151(11):6470-81.
Identification of membrane antigen C33 recognized by monoclonal antibodies - Identification of membrane antigen C33 recognized by monoclonal antibodies
Fukudome K, Furuse M, Imai T, Nishimura M, Takagi S, Hinuma Y, Yoshie O. J Virol. 1992 Mar;66(3):1394-401.