CREB (48H2) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 9197
• Prices: $99.00, $256.00, $637.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: 48H2
• Applications: WB IP IHC-P IHC-F ICC/IF FC ChIP
• Species Reactivities: Human, Mouse, Rat, Monkey, Drosophila
• Antigen: Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CREB-1 protein.
• Description: Rabbit Monoclonal
• Gene: CREB1

Product images

Western Blot analysis of extracts from SK-N-MC, COS, NIH/3T3, C6 and Drosophila S2 cells, using CREB (48H2) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human astrocytoma, using CREB (48H2) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using CREB (48H2) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, using CREB (48H2) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded mouse brain, using CREB (48H2) Rabbit mAb.

Immunohistochemical analysis of frozen H1650 xenograft, showing nuclear localization using CREB (48H2) Rabbit mAb.

Confocal immunofluorescent analysis of mouse cerebellum labeled with CREB (48H2) Rabbit mAb (red) and Neurofilament-L (DA2) Mouse mAb #2835 (green). Blue pseudocolor =DRAQ5® #4084 (fluorescent DNA dye).

Confocal immunofluorescent analysis of SK-N-MC cells showing nuclear stain with CREB (48H2) Rabbit mAb (A, red) compared to an isotype control (B). Blue pseudocolor =DRAQ5® (fluorescent DNA dye).

Flow cytometric analysis of SK-N-MC cells using CREB (48H2) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 293 cells treated with Forskolin #3828 (30 μM) for 1h and 10 μl of CREB (48H2) Rabbit mAb, using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared from 5 ng enriched ChIP DNA using NEBNext ® Ultra™ II DNA Library Prep Kit for Illumina ® , and sequenced on the Illumina NextSeq. The figure shows binding across NR4A3, a known target gene of CREB (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 293 cells, treated with Forskolin #3828 (30 μM) for 1h and either 10 μl of CREB (48H2) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP ® Human NR4A3 Promoter Primers #4829, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.