Anti-TATA binding protein TBP antibody [mAbcam 51841] - Nuclear Loading Control and ChIP Grade (HRP)

• Supplier: Abcam
• Catalog: ab197874
• Prices: $425.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone: mAbcam 51841
• Applications: WB IHC-P
• Species Reactivities: Human, Mouse, Rat, Bovine, Xenopus, Chimpanzee, Zebrafish
• Antigen: Synthetic peptide corresponding to Human TATA binding protein TBP aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH)
• Description: Mouse Monoclonal
• Gene: TBP
• Conjugate: HRP

Product images

IHC image of TATA binding protein TBP staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab197874, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

All lanes : Anti-TATA binding protein TBP antibody [mAbcam 51841] - Nuclear Loading Control and ChIP Grade (HRP) (ab197874) at 1/5000 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab150036)Lysates/proteins at 20 µg per lane.developed using the ECL techniquePerformed under reducing conditions.