Anti-TATA binding protein TBP antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab28175
• Prices: $392.00
• Sizes: 200 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ChIP
• Species Reactivities: Mouse, Human
• Antigen: Recombinant full length TATA binding protein TBP (Human)
• Description: Rabbit Polyclonal
• Gene: TBP
• Conjugate: Unconjugated

Product images

ChIP analysis using ab28175 binding TATA binding protein TBP in mouse liver nuclear tissue lysate. Cells were cross-linked for 10 minutes with 1% paraformaldehyde. Samples were incubated with primary antibody for 16 hours at 4°C in TE pH8, 150mM NaCl, Trition 1%, SDS 0,1%. Protein binding was detected using real-time PCR.Negative Control: beads.See Abreview

Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 8µg of  ab28175 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located either in the core promoter of the gene (TATA) or in the open reading frame (ORF).

Product References

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