All lanes : Anti-TBLR1 antibody [EPR16153] (ab190796) at 1/1000 dilutionLane 1 : K562 cell lysateLane 2 : Hela cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-TBLR1 antibody [EPR16153] (ab190796) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : Raw 264.7 cell lysateLane 3 : PC-12 cell lysateLane 4 : NIH-3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostatic hyperplasia tissue, labeling TBLR1 with ab190796 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse muscle tissue, labeling TBLR1 with ab190796 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde, labeling TBLR1 with ab190796 at 1/200 dilution. Goat anti rabbit IgG(Alexa Fluor® 555) was used as the secondary antibody at 1/200. Counterstained with DAPI.
Immunofluorescence analysis of PC-3 cells fixed in 4% paraformaldehyde, labeling TBLR1 with ab190796 at 1/200 dilution. Goat anti rabbit IgG(Alexa Fluor® 555) was used as a secondary antibody at 1/200. Counterstained with DAPI.