Anti-TBLR1 antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab24550
• Prices: $392.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ChIP IP WB ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Recombinant full length protein (Human)
• Description: Rabbit Polyclonal
• Gene: TBL1XR1
• Conjugate: Unconjugated

Product images

Anti-TBLR1 antibody - ChIP Grade (ab24550) at 1/2000 dilution + HeLa nuclear extract

ab24550 used in Immunoprecipitation.Lysate prepared from human lung carcinoma cells was loaded at 500µg. The immunoprecipitation step was performed using Protein G. 50% of the immunoprecipitated material was loaded on each lane. Membrane was blocked with blocking buffer (PBS+ 5% non fat milk powder) for 1 hour at room temperature followed by incubation with ab24550 in blocking buffer plus 0.1% Tween-20 overnight (around 16 hours) at 4 degree centigrade. Blot was washed thrice in PBS plus 0.1% Tween-20, followed by incubation with anti-rabbit IgG HRPO in blocking buffer plus 0.1% Tween-20 at room temperature for 1 hour. Blot was washed thrice in PBS plus 0.1% Tween-20, followed by ECL detection of reactive bands.See Abreview

Product References

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