Anti-TBR2 / Eomes antibody - ChIP Grade
| Name | Anti-TBR2 / Eomes antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab23345 |
| Prices | $401.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F IHC-F WB ICC/IF ICC/IF IHC-P ChIP ChIP ChIP IHC-F |
| Species Reactivities | Mouse, Rat, Human, Marmoset, Bovine |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Mouse TBR2/ Eomes |
| Description | Rabbit Polyclonal |
| Gene | EOMES |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-TBR2 / Eomes antibody - ChIP Grade (ab23345) at 1 µg/ml (BLOCKED WITH 3% MILK)Lane 1 : Human Mesendoderm (Day 2) Whole Cell LysateLane 2 : E14 Mouse Embryo Brain Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutionPerformed under reducing conditions.
Lanes 1 - 3 : Anti-TBR2 / Eomes antibody - ChIP Grade (ab23345) at 1/2000 dilutionLanes 4 - 6 : V5 antibodyLane 1 : EL4 cells + empty vectorLane 2 : EL4 cells + vector expressing V5 tagged Eomesodermin Lane 3 : EL4 cells + V5 tagged vectorLane 4 : EL4 cells + empty vector Lane 5 : EL4 cells + vector expressing V5 tagged EomesoderminLane 6 : EL4 cells + V5 tagged vectorSecondaryGoat anti Rabbit at 1/2500 dilution
ab23345 staining mouse developing cerebral cortex tissue sections by IHC-Fr. Sections were PFA fixed and permeabilized in TX-100 prior to blocking with 2.5% serum for 1 hour at RT. The primary antibody was diluted 1/500 and incubated with the sample for 18 hours. A biotinylated pig anti-rabbit IgG antibody, diluted 1/500, was used as the secondary.See Abreview
All lanes : Anti-TBR2 / Eomes antibody - ChIP Grade (ab23345) at 1 µg/mlLane 1 : Human Mesendoderm (Day 2) Whole Cell Lysate Lane 2 : Human Mesendoderm (Day 2) Whole Cell Lysate with Mouse TBR2 / Eomes peptide (ab25698) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
Human embryonic stem cells were differentiated into mesendoderm. Cells were then stained with DAPI (blue) and a TBR2 / Eomes specific antibody, ab23345 (1:100, red). Ludovic Vallier, University of Cambridge
ab23345 staining TBR2 / Eomes in mouse embryonic brain tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and blocking with 1% BSA and normal Goat serum for 30 minutes at RT. The sample was incubated with primary antibody (1/1000 in TBS + BSA 1%) for 10 hours at 40C. An Alexa Fluor® 555-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/800 dilution.See Abreview
ab23345 at a 1/500 dilution staining TBR2/Eomes in E18 mouse brain tissue sections, by Immunohistochemistry/ PFA, incubated for 16 hours at 4°C. Permeabilized using 0.1% Triton in PBS. Blocked with 10% serum for 1 hour at 25°C. Secondary used undiluted polyclonal donkey anti-rabbit conjugated to Alexa Fluor 555 (green). DAPI staining (blue).See Abreview
Anti-TBR2 / Eomes antibody - ChIP Grade (ab23345) at 1/1000 dilution + Lysate prepared from mouse embryonic brain tissue at 20 µgSecondaryHRP-conjugated goat polyclonal to rabbit IgGdeveloped using the ECL techniquePerformed under reducing conditions.
IHC-FoFr image of TBR2 staining in 8 week old mouse hippocampus using ab23345 (1.5µg/ml). The animal was perfusion fixed using 4% PFA. the tissue harvested and postfixed for 24 hours. The tissue was then cryoprotected in 30% Sucrose for 48 hours. The tissue was sectioned at 45µm thickness using a cryotstat and staining performed using 'free floating technique'.
Product References
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