Immunofluorescence of A2058 cells labelling TBX1 with ab103127. A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab103127 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TBX1 immunoreactivity is localized to the nucleus.
Formalin-fixed and paraffin-embedded Human testis tissue, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining, using ab103127 at a dilution of 1/50.
Anti-TBX1 antibody (ab103127) at 1/100 dilution + HepG2 cell lysate at 35 µg