Anti-TCEA1 antibody (ab76637) at 1/500 dilution + Recombinant fragment of human TCEA1 at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
All lanes : Anti-TCEA1 antibody (ab76637) at 1/500 dilutionLane 1 : TCEA1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
The antibody concentration should be 10µg/mL in coating buffer (PBS), the volume should be 100µL/well. Blocking solution was added at 250ul per well (5% Skim Milk in PBS + 0.05% Tween-20). Secondary antibody used was HRP conjugated goat anti-rabbit IgG (H+L).