All lanes : Anti-TCEA1 antibody [EPR14820(B)] (ab184181) at 1/10000 dilutionLane 1 : K652 lysateLane 2 : HeLa lysateLane 3 : SP2/0 lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunoprecipitation analysis of HeLa cell lysate labeling TCEA1 with ab184181 at 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated used as secondary antibody at 1/1000 dilution.
Immunohistochemistry analysis of Human lung adenocarcinoma tissue labeling TCEA1 with ab184181 at 1/100 dilution. Secondary antibody HRP conjugated Rabbit IgG. Counterstained with Hematoxylin.
Immunohistochemistry analysis of Human colon tissue labeling TCEA1 with ab184181 at 1/100 dilution. Secondary antibody HRP conjugated Rabbit IgG. Counterstained with Hematoxylin.
Immunocytochemistry analysis of a HeLa cells labeling TCEA1 with ab184181 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®488) at a 1/200 dilution. Dapi counterstain shown in blue.