Anti-TCEA1 antibody [EPR14821]

• Supplier: Abcam
• Catalog: ab185947
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR14821
• Applications: WB IHC-P ICC/IF ICC/IF FC IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TCEA1 aa 200-300
• Description: Rabbit Monoclonal
• Gene: TCEA1
• Conjugate: Unconjugated

Product images

All lanes : Anti-TCEA1 antibody [EPR14821] (ab185947) at 1/10000 dilutionLane 1 : K562 cell lysateLane 2 : HeLa cell lysateLane 3 : SP2/0 cell lysateLane 4 : HUVEC cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin embedded Human bladder transitional cell carcinoma tissue labeling TCEA1 with ab185947 at 1/500 dilution. Counterstained with Hematoxylin.

Immunofluorescent staining of -20 °C acetone fixed HeLa cells labeling TCEA1 with ab185947 at 1/500 dilution (red; left image) and  DAPI nuclear staining (blue; right image).  Secondary staining with Goat anti Rabbit IgG (Alexa Flour®555) at a 1/200 dilution.

Immunofluorescent staining of 4% paraformaldehyde fixed HUVEC cells labeling TCEA1 with ab185947 at 1/500 dilution (red; left image) and  DAPI nuclear staining (blue; right image).  Secondary staining with Goat anti Rabbit IgG (Alexa Flour®555) at a 1/200 dilution.

Flow cytometric analysis of 2% paraformaldehyde HeLa cells labeling TCEA1 with ab185947 at 1/250 dilution (red) compared to a Rabbit monoclonal IgG isotype control antibody (green).  Secondary staining with Goat anti Rabbit IgG (FITC) at a 1/150 dilution.

Immunoprecipitation analysis of HeLa cell lysate labeling TCEA1 with ab185947 at 1/50 dilution.  Secondary staining with Goat anti Rabbit IgG (H+L) Peroxidase conjugated antibody at a 1/1000 dilution.