![All lanes : Anti-TCEA1 antibody [EPR15036] (ab184158) at 1/50000 dilutionLane 1 : Jurkat cell lysateLane 2 : 293 cell lysateLane 3 : K562 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/9130_ab184158-216480-ab184158WB.jpg)
All lanes : Anti-TCEA1 antibody [EPR15036] (ab184158) at 1/50000 dilutionLane 1 : Jurkat cell lysateLane 2 : 293 cell lysateLane 3 : K562 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescence analysis of paraformaldehyde-fixed HUVEC cells, staining TCEA1 (red) with ab184158 at 1/250 dilution. Alexa Fluor®555-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate, labeling TCEA1 immunoprecipitated using ab184158 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.