Immunofluorscence analysis of HUVEC cells labeling TCEA1 with ab184159 at 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution (left). Counter-stained with Dapi (right).
Immunoprecipitation of Jurkat lysate labelling TCEA1 with ab184159 at 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody used at 1/1000 dilution
All lanes : Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159) at 1/50000 dilutionLane 1 : Jurkat lysateLane 2 : K562 lysateLane 3 : 293 lysateLane 4 : HUVEC lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution