Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121231 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-TCP11L1 antibody (ab121231) at 1/250 dilutionLane 1 : Negative control (vector only transfected HEK293T lysate) Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in HEK293T cells
ab121231, at 1/100 dilution, staining TCP11L1 in Human lymph node tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue. Note: Shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.